Molecular testing powered by Pulse Controlled Amplification (PCA)
10,000 times faster temperature ramps
Conventional nucleic acid amplification platforms rely on thermocyclers which
limit the speed of the reaction. Pulse Controlled Amplification (PCA) uses microcyclers (micro or nano-scale thermal cyclers) that are immersed in an amplification solution containing standard reagents and target sequences. PCA microcyclers are selectively heated by an external energy source and are instantaneously cooled by the surrounding reaction solution, resulting in temperature ramps more than 10,000 times faster than conventional nucleic acid amplification.
Nucleic acid amplification in minutes
Microcyclers convert externally applied energy pulses into heat in a melting zone located directly on their surface, causing denaturation of covalently attached nucleic acids. Following denaturation, the solution is held at constant temperature for annealing and elongation. Repeated energy pulses control the speed of amplification cycles. PCA reaction times are typically 10 minutes or less.