Pulse Controlled Amplification (PCA) – A platform technology for ultrafast PCR

Conventional PCR platforms rely on thermocyclers that limit the speed of the reaction. Pulse Controlled Amplification (PCA) is a breakthrough platform technology developed by GNA Biosolutions.

Microcyclers act as thermal hubs for the PCR reaction
PCR-enabled microcyclers (micro or nano-scale thermal cyclers) are immersed in a solution containing standard PCR reagents (polymerase, dNTPS, magnesium etc) and target sequences. At the beginning of the reaction, the solution is heated to melting temperature to denature target sequences, and then held at constant temperature. Denatured target sequences anneal to primers on the surface of microcyclers and elongation occurs at the same temperature.

 

Energy pulses control the speed of nucleic acid amplification
For denaturation, microcyclers are selectively targeted by an external energy source. Microcyclers absorb energy, which is converted into heat in the melting zone located directly on their surface. Microcycler heating causes denaturation of nucleic acids in the melting zone. Microcyclers are instantaneously cooled by the surrounding reaction solution, resulting in temperature ramps a million times shorter than conventional PCR. Repeated energy pulses control microcycler heating, and consequently the speed of amplification cycles.

 

Laser PCR®

Laser PCR® is a PCA technology designed for time-sensitive molecular applications. In Laser PCR®, a high-powered laser is used to selectively heat PCR-enabled gold nanoparticles in solution. Repeated laser pulses control the speed of nucleic acid amplification, with real-time results in 10 minutes or less.

 

GNA Biosolutions launched its first Laser PCR instrument, the Pharos V8 in 2017. To learn more about the Pharos V8 or to schedule a demo, please contact us at

 

 

Universal Assay

The Universal Assay lets you create your own assays in-house, without the limitation of a fixed menu. In Laser PCR®, gold nanoparticle thermal cyclers are PCR-enabled through the addition of a universal adapter, which binds to an anchor sequence on PCR primers. Primers annealing to target sequences anchor nascent amplicons to the nanoparticles.

 

PCR-enabled nanoparticles are provided as part of the Universal Assay Kit. Just add an anchor sequence to your existing primers and you’re ready to run your own ultrafast assays, with real time results in 10 minutes or less.