Laser PCR®: Lighting the way for Innovation

Laser PCR® is a patented molecular technology, based on Pulse Controlled Amplification,  developed by GNA Biosolutions and designed for time-sensitive molecular applications.

 

Limitations of conventional PCR

Conventional Polymerase Chain Reaction (PCR) requires time-consuming, repetitive heating and cooling cycles, typically achieved by a heating block or a stream of air. The poor thermal conductivity of the bulk solution and reaction vessel severely limits heating and cooling rates in conventional PCR, and increases time to result.

Conventional Heating

Laser heating of nanoparticles

In Laser PCR®, gold nanoparticles are conjugated with nucleic acids and added to a PCR reaction containing a potential target. A laser beam is pulsed through the solution, causing the nanoparticles to absorb light and emit heat within a short range, creating cycles of denaturation, annealing, and elongation.

1.000.000x shorter PCR temperature ramps

Heating is localized to the nanoparticles while the bulk solution is held at constant temperature. This allows heating and cooling ramps a million times faster than conventional PCR and accelerates time to results.

10x faster Cycles

 

Ultrafast time to results

Realizing the potential of PCR-based molecular applications requires time-sensitive approaches. By achieving ultrafast time to results, Laser PCR® creates new possibilities for molecular applications in point of care testing, infectious diseases, biosecurity and food safety. Laser PCR® accelerates time to results, which can accelerate time to decisions and help deliver the power of molecular technology to multiple industries and environments.

A variety of detection options are possible with Laser PCR®, including real-time fluorescence detection of hydrolysis probes within 10 minutes.

 

 

Watch the Laser PCR® video