Header Technology

GNA’s breakthrough technology
Laser PCR® with local heating

We employ a radically new approach to PCR, with heating
and cooling rates that are many orders of magnitude
faster than any PCR so far. This is achieved by
adding laser heated nanoparticles to the solution,
which function as the world’s smallest thermal cyclers.
This local heating technology drives GNA’s new
PCR amplification (Laser PCR®) and fluorescent-free
DNA detection (NANOSTOVE®).

Conventional Heating

Superior to conventional PCR

Heating and cooling has been the bottle-neck of Polymerase Chain Reaction (PCR) since its invention about thirty years ago. All approaches so far require time-consuming, repetitive heating and cooling of the entire sample. Usually, this is achieved by a heating block or a stream of air that causes a heat transport in and out of the sample. However, the poor thermal conductivity of the PCR solution as well as of the reaction vessel severely limit the heating and cooling rates in conventional PCR.

10x faster PCR cycles

Laser PCR® enables heating and cooling ramps on a microsecond time scale. After the elongation of the primers, a laser pulse locally heats up the nanoparticles, thereby denaturing the newly formed DNA double strand. As the reaction remains at the productive temperature of the polymerase virtually the entire time, the cycle duration can be kept as low as 3 seconds. Laser PCR® allows us to have 1000000x shorter PCR temperature ramps by laser-heating of nanoparticles in comparison to the conventional PCR with its global heating process. Overall, the PCR cycles with Laser PCR® are about 10 times faster.

10x faster Cycles

See how it works in this animation (no sound)


Real-Time detection with fluorescent probes

While a variety of read-out options are possible, fluorescence-based detection of hydrolysis probes in real-time works perfectly with Laser PCR®.